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After 48 h, cells were pelleted at 2000 g and resuspended in breaking buffer (50 mM sodium phosphate, 5% glycerol, 1 mM EDTA, pH 7.4) and frozen prior to processing

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Virus-infected Sf9 cells were harvested, their membranes were isolated and stored, and the membrane protein concentrations were determined as described, with minor modifications(Bakos et al

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