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Inside the cell, the -glutamyl amino acid is hydrolyzed by -glutamyl cyclo-transferase and converted into oxoproline and a free amino acid

Vitamin B12 Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Vitamin B12 in biofluid samples, such as serum, plasma, tissue homogenates and other biological fluids. This is a competitive inhibition Sandwich ELISA in strip well format with reagents for up to 96 tests . VB12 present in samples reacts with an anti-VB12 monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, VB12 conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active VB12 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of VB12. Detection Target: Vitamin B12 Reactivity: Multi-species Range: 4.68-300ng/mL Sensitivity: 3.8ng/mL Storage Conditions: Store all components at 4C This Multi-species VB12 activity assay is for the quantitative determination of Vitamin B12 in samples including serum, plasma, tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results

Then, cells were stimulated with 10 M (final concentration) forskolin for 10 min in the dark at room temperature