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Materials and Methods Principle of developed redox profiling method For quantitative analysis of NAD + , NADH, NADP + , NADPH, GSSG and GSH in a single blood sample, we developed a comprehensive method, NADMED, consisting of novel extraction coupled with detection based on previously published cyclic enzymatic reactions with colorimetric detection 23,24,3234 in a high-throughput 96-well-plate analysis

To break-out the region displaced by the ligand, the grid_comp command was run on the results of the previous step

However, the function and mechanism of GPX3 in breast cancer have not been reported and need to be further elucidated