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Meanwhile, the results were stored in the FASTQ (abbreviated as fq) file format, which contains the sequence information of the sequencing sequences (Reads) and their corresponding sequencing quality information

and our targeted LCMS data demonstrate a profound change in broad metabolic activity in cells, supported by RNA-seq analysis of metabolic gene expression changes

In fact, healthy individuals with a high genetic risk for IBD exhibit lower abundance of Roseburia spp., a major butyrate-producing genus, implying that dysbiosis may precede clinical disease onset (Imhann et al., 2018)

The cells were stained with 100 g/mL of propidium iodide (Pi) and treated with 100 g/mL RNase A for 30 min