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Starting at ZT1 (1 h post lights on), the most fluorescent nematodes were selected manually under a SMZ100 stereomicroscope equipped with an epi-fluorescence attachment (Nikon, Minato, Tokyo) with a cool Multi-TK-LED light source (Tolket S.R.L., Buenos Aires, Argentina) to avoid warming the plate

Embryogenesis involves a series of differentiation and maturation processes that are well balanced by spatial and temporal controls of cellular gene expression involving mechanisms on epigenetic, transcriptional, and post-transcriptional levels according to an inbuilt biological program (Ufer et al., 2010)

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Smokers may suffer chest pain after smoking, but certain pains may be signs of a more serious issue