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For the proteomic analysis, 300 L aliquots of urine were centrifuged twice at 10,000 g for 20 min at 4C to separate urinary proteins from small molecules using an Amicon Ultra 0.5 ml Centrifugal Filter (10 kDa, UFC501096, Merck Millipore, Darmstadt, Germany) according to the manufacturers instructions

There are many naturally occurring antioxidants, each with its own unique composition, physical and chemical characteristics, mechanisms, and action sites 25

Herbal infusions of black seed and wheat germ oil: Their chemical profiles, in vitro bio-investigations and effective formulations as Phyto-Nanoemulsions

Plant Foods Hum Nutr 56:349358