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Physico-chemical characterization of nanocarriers The average size, polydispersity index (PDI) and surface charge (zeta potential) of nanocarriers were measured by dynamic light scattering (Malvern Zetasizer Nano ZS, equipped with a HeNe laser ( = 632.8 nm), Malvern Instruments, UK)

The molecular gutliver signaling, we substantially revised the manuscript with detailed discussions of key pathways: TLR4/NF-B, MAPK signaling, tight junction regulation, microbial metabolite signaling (e.g., SCFAs), and cytokine cascades

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Getting enough of this vitamin is especially important for maintaining a healthy immune system