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Key to the effectiveness of PAE is identifying the right candidates by confirming the BPH diagnosis and ruling out other causes such as muscle or bladder dysfunction, prostatitis or prostate cancer

These timepoints were selected to represent distinct stages of iHLO development and maturation: Day 7 signifies the hepatoblast differentiation stage, Day 15 marks the early hepatocyte stage, and Day 30 and Day 45 indicate progressively matured liver organoid stages with increasing liver cell populations and functions

Their reactivity is based on the 1,3-dicarbonyl chemistry originally demonstrated with dimedone

Cells in 6-well plates were labeled with 2 M BODIPY for 30 min, trypsinized, strained, resuspended in FACS buffer, and analyzed by flow cytometry (FITC/Texas Red channels on a BD LSRFortessa TM cell analyzer)