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The rich blood supply, allows medications to be absorbed faster through muscle fibres than through the subcutaneous route (Malkin, 2008

Inserting the needle smoothly at the appropriate angle (90 degrees for IM, 45-90 degrees for SC depending on subcutaneous tissue thickness)

Brains were quickly extracted and sliced (300 m) for whole cell recordings in current-clamp and voltage-clamp mode

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