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Baskin reported a normal anatomy of the hypospadiac penis except on its ventral aspect with the possibility of biological and structural defects [24]

Live and dead cell staining Live and dead cells were distinguished using the Calcein-AM and propidium iodide (PI) doubling staining method, according to the instructions provided by the manufacturer (Solarbio, Beijing, China)

Hypoxic stimulation causes the tumor to oversecrete proangiogenic factors and induce angiogenesis (Potente et al , 2011)

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