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GST-agarose was added to lysis buffer, incubated for 4 h at 4 C, washed four times with lysis buffer and analyzed by immunoblotting

FIGURE 5 In addition to the canonical -lyase reaction, CSE also catalyzes -elimination reactions with various substrates including cysteine and cystine ( in vivo generation of H 2 S and CysSSH from cysteine and cystine, respectively