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Under these conditions, it may help to add a stretch of glycines, or histidine or a flag tag between the TEV cleavage site and the N-terminus of the target protein

Secher A, Jelsing J, Baquero AF, Hecksher-Sorensen J, Cowley MA, Dalboge LS, et al

Bodykhov, et al., Zh

The results showed that Malg not only significantly improved the symptoms of inflammatory malady, such as weight loss and systemic inflammatory response, in the AIA model, but also outperformed conventional hydrocortisone (HC) treatment in reducing arthritis scores (109)