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Littermates of the same sex were randomly assigned to experimental groups and were fed a chow diet or a Western diet (ssniff, TD88137) with or without 10% fish oil (ssniff supplementation, see Table S2)

Its deletion increases mitochondrial biogenesis and UCP-1 expression, reduces autophagy flux, and promotes WAT browning, indicating that E2F1 suppresses oxidative capacity by maintaining basal mitophagy

However, keep in mind: Results are not guaranteed Effects vary widely between individuals Benefits are generally subtle rather than dramatic Consistent use is most important GHK-Cu should be viewed as a supportive ingredient, not a primary treatment for aging or hair loss

Unlike topical treatments that may show results in weeks, systemic glutathione typically requires several months of regular use before significant improvements in hyperpigmentation become visible