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The resuspended bacteria were lysed by ultrasonication on ice (four 5-min sessions with 30% amplitude, 2.0 s pulse on, 2.0 s pulse off, interspersed with 1-min pauses)

A ROC curve was generated for a validation set of six WSIs by applying a series of thresholds between 0-1, resulting in an AUC value of 0.92 and a balanced accuracy value of 0.87 at the optimised threshold

RT-PCR evaluation of striatal TNFR-1, HMGB-1 (high-mobility group box 1), and TLR4 From the tissue lysate, total RNA was separated employing RNeasy-Mini-kit (Qiagen/Venlo/Netherlands), and its purity was assured at OD 280/260 nm spectrophotometrically

In sunflower axes of embryos treated with HCN, CAT and SOD activities were inhibited (while the GR activity was not affected) (Table 1) (Oracz et al