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Some strains were obtained by crossing

Cell culture and treatment FL83B mouse hepatocytes (ATCC , Manassas, VA) were cultured and maintained in F-12K complete medium

Hagen I, Ecker M, Lagorce A, Francois JM, Sestak S et al (2004) Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins