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4.5 Live cell imaging Confocal images of 4 optical planes interspaced by 1 m (from -1.0 m to 2.0 m included) across 25 x 25 fields of view with a 10% overlap were acquired per well on an Opera Phenix High Content Screening System (PerkinElmer) using a 40x or 63x/1.15 NA water-immersion objective, with live cell parameters (37 C and 5% CO 2 )

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