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Unfortunately, head trauma often disrupts normal sleep patterns, creating a vicious cycle where lack of sleep slows recovery, which prolongs sleep disorders and impedes concussion rehabilitation progress

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The PCR conditions were adjusted according to the kit protocol as follows: reverse transcription for 10 min at 45 C, Polymerase activation for 2 min at 95 C, denaturation (40 cycles) for 5 s at 95 C, and annealing/extension for 20 s at 60 C

Mitchell KG, Parra ER, Nelson DB, Vaporciyan AA, Hofstetter WL, Mehran RJ, Swisher SG, Rice DC , Sepesi B, Walsh GL, Zhang J, Behrens C, Kalhor N, Weissferdt A, Cesar M, Wistuba I, Fujimoto J, Roth JA, Antonoff MB