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GST-Pull Down Technology Introduction to protein interactions The functioning of biological systems is inextricably linked to protein-protein interactions, and important biological processes such as DNA synthesis, gene transcriptional activation, protein translation, modification and localization, and information transfer all involve the role of protein complexes

The mixture was combined with an assay mixture of Amplex Red reagent (20 mM stock to 0.1 mM) and HRP (200 U/ml stock to 0.2 U/ml) and added to the wells at 50 l/well

10.1038/s41698-024-00757-w 14 FurugakiK.FujimuraT.SakaguchiN.WatanabeY.UchiboriK.MiyauchiE.et al (2025)

In fact, gonadotropin-releasing hormone (GnRH) stimulates gonadotroph cells to produce pulsatile LH and FSH