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These variations support their implications as experimental tools for probing ligand-specific signaling bias, receptor desensitization, and regulatory feedback mechanisms within ghrelin axis biology

The plate was then spun at 2700 x g for 3 min using a plate holder centrifuge, the supernatant was discarded, and the cells were washed 1X with PBS for fixing, as described earlier, before analysis by flow cytometry (two-step), or for the chase step with an azido-tagged dye (three-step), as described earlier

Boxmeer JC, et al

Unbound sugar was removed by extensive dialysis against distilled water and this Amadori-rich protein reincubated with or without the prospective extract at 37 C