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Transepidermal pathway The movement of a permeant across the skin occurs through both intracellular and extracellular spaces along the transepidermal pathway, starting from the epidermis and progressing to the dermis and then the hypodermis

According to the latest guidelines from the American Veterinary Medical Association (50) and guidelines adopted by institutions such as Boston University (51) and the University of Maryland (52), the rats were anesthetized by intraperitoneal injection of ketamine (80 mg/kg) and xylazine (10 mg/kg), in compliance with approved ethical standards (53,54)

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A review published in Frontiers in Cellular and Infection Microbiology indicated that antimicrobial peptides are used as pharmacological agents to modulate the immune response, promote wound healing and prevent post-surgical adhesions