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The mobile phase consisted of 0.1% formic acid in water (A) and methanol (B), with a gradient elution: 2% B to 30% B over 30 min, then to 60% B in 8 min, to 100% B in 2 min (hold 5 min), and re-equilibration to initial conditions in 1 min

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Flow-through particles were initially developed in the early 1990s for ion-exchange chromatography and were later adapted for use in affinity chromatography