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Description

Glutaminase (GLS) generates glutamate via glutaminolysis to support anaplerosis but generates more ammonia ( Fig

For the generation of 3XHA-EGFP-OMP25 (HA-Mito) retrovirus 47 , Phoenix-AMPHO 293 T cells (ATCC, CRL-3213) were cultured in DMEM supplemented with 5% FBS to 90% confluency

In addition, we have selected combinations of mutants that could affect the interaction with targets but not compromise the thermal stability of the CHC-NTD fold, as predicted by FoldX 5 61 using a threshold of 1 kcal/mol