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ROS are characterized as either radicals with at least one unpaired electron or reactive nonradical compounds that can oxidize biomolecules

mPEG-MAL binds to every free Cys and alters the mobility of the protein on a non-reducing SDS-PA gel electrophoresis (PAGE) depending on how many thiol-mPEG conjugates are formed

This functional difference is further reflected in clonogenicity assays, where Tie2 + NPPCs form spheroid colonies in 3D culture, while Tie2 cells generate spread, fibroblastic colonies [42, 43]