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To prepare hippocampal slices, horizontal sections (400 m thick) at the middle septotemporal levels were cut in ice-cold cutting solution using a Leica VT1000S vibratome (Leica Microsystems) and allowed to recover at 32 C for at least 1 h in an oxygenated (95% O 2 /5% CO 2 ) artificial cerebrospinal fluid (aCSF) solution (124 mM NaCl, 2 mM KCl, 2 mM MgSO 4 , 2 mM CaCl 2 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 , and 10 mM Glucose, with pH 7.3 and osmolarity 300 mOsm/L)

Strmstedt further demonstrated that CYP51A1 is widely expressed in human tissues, with highest levels in steroidogenic organs such as the testis, ovary, adrenal gland, liver, and prostate

DEHP, di-(2-ethylhexyl) phthalate

There may also be some pressure at the insertion site during the procedure