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Mitochondrial Ca 2+ Uptake/Retention Capacity Mitochondrial calcium uptake/release was measured by following the fluorescence changes in the medium containing 0.1 M calcium green-5N hexapotassium salt using a spectrofluorometer (Hitachi F-4500, Japan) operating at 506 nm for excitation and 531 nm for emission (Ronchi et al., 2013)

Disruption of this barrier permits microbial translocation into the submucosa, triggering inflammatory cascades that contribute to the pathogenesis of intestinal disorders such as IBD (159)

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