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The medium was inoculated at an optical density of 0.2 (at 600 nm) and the cultures were incubated at 25 C, without shaking

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To address this, researchers have investigated the toxic impact of NAg on environmental organisms, plant and animal models, and human cells, with studies still on-going to determine the toxicity threshold and long-term environmental and human health effects of NAg (Burduel et al., 2018