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Fluid Phase Equilibria 285(12), 9095.10.1016/j.fluid.2009.07.017CrossRefGoogle Scholar Tyunina, EY et al

A limitation of this method is that it requires the treatment of protein under anaerobic conditions, as TNB 2 readily converts to DTNB (Ellmans reagent) under aerobic conditions, leading to false positive identifications from free thiol labeling