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There are three fundamental mechanisms of disease known to produce EDS: deficiency of collagen-processing enzymes, dominant-negative effects of mutant collagen -chains, and haploinsufficiency

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The plate was incubated at 37 C for 1 min followed by another 1 min wherein it was mixed and the fluorescence ( ex / em = 488/518 nm) recorded every 2 min for the duration of the experiment