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Description

10.7759/cureus.15208 Raed Atiyat, Riyashat Kazmi, Krunal Trivedi, Hamid S Shaaban

Beghi E, Bendotti C, Mennini T

In laboratory and preclinical research settings, 5-Amino-1MQ is used to investigate metabolic efficiency, fat storage mechanisms, and energy utilization pathways, making it a compound of interest in metabolic and body composition research

The proteins were precipitated again with 10% TCA, washed twice with acetone and the pellet resuspended in alkylation buffer (2% SDS, 100 mM Tris pH 8.0 containing 5 mM malPEG-2000 (NOF Corporation, Japan)) at 37C for 1 hour