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All assays were performed according to the manufacturers protocols

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Although the function of LRRK2 has not been completely elucidated, studies have shown that the protein is involved in regulation of mitochondrial dynamics and mitochondrial quality control ( LRRK2 cause the late-onset autosomal dominant form of PD, which represents the most common familiar PD, see LRRK2 mutations show clinical traits and pathological features indistinguishable from those of sporadic PD cases ( LRRK2 missense mutations have confirmed pathogenicity, including G2019S, Y1699C, R1441C/G/H, N1437H, I2020T, G2385R, and R1628P, see Increasing evidence has confirmed the key role of LRRK2 in mitochondrial function

As such, NAM can exert end-product inhibition of these enzymes