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Apply light pressure with a clean cotton ball if any bleeding occurs

Luo N, Nixon MJ, Gonzalez-Ericsson PI, Sanchez V, Opalenik SR, Li H, Zahnow CA, Nickels ML, Liu F, Tantawy MN

(2013) found that a pretreatment with water at 45C followed by treatment with 0.05 M NAC and CaCl 2 , resulted in a greater reduction in browning

Slices were cultured in Minimum Essential Medium (MEM) with Hanks balanced salts [Thermo Fisher Scientific, #11575032], 1% penicillinstreptomycin and 25% horse serum [Thermo Fisher Scientific, #16050130] for 3 days, with 1 mL of medium added below the membrane insert