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Acta Cir Bras

Proteins were detected with the indicated antibodies listed in the Antibody table, and blots were imaged using Amersham Imager 680 (GE HealthCare, USA)

This analysis identified that lysine 133 (K133) of IL-1 was targeted for ubiquitylation (marked by a Lys--Gly-Gly (diGly) linkage), while the adjacent serine (S134) was phosphorylated (Fig