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Real-time PCR was performed as previously described [54] using 10 ng cDNA to determine gene expression levels using primers EBI11 (5-CGCTACGGCAAGACCATG-3) and EBI12 (5-GCGCTCCAACTGGCTCGG-3) for gshA , BT5458 (5-CGCATGCGCCCGCTGAAGG-3) and BT5459 (5-GCGCGCCAGGCAGTAGGG-3) for gshB , EBI315 (5-CGGTCAGCGGTACAGGGAA-3) and EBI316 (5-GCGAGAGTACCAACGGTTGAAA-3) for phzA1 , and EBI316 and EBI317 (5-CGTCGCACTCGACCCAGAA-3) for phzA2