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Corpus callosum tissue was isolated from the brains of differently treated mice and sampled by homogenization in RIPA lysis buffer (Beyotime Biotechnology) supplemented with a protease inhibitor (Sigma)

Ascorbic Acid (Vitamin C) Injection is primarily used to treat or prevent vitamin C deficiency in patients unable to take oral supplements, or in clinical situations where higher plasma concentrations are required, such as certain infections, wound healing, or adjunctive therapy in specific protocols

With this technique, sperm are frozen for later use with in vitro fertilization or intrauterine insemination

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