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Sperm apoptosis and necrosis The distribution of viable, apoptotic, and necrotic spermatozoa following cryopreservation is presented in Table 2

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Cells were exposed to a 1:300 dilution of the primary antibody anti-xCT, in blocking solution overnight at 4 C, followed by the incubation with fluorescein-labeled goat anti-rabbit (1:1000) antibodies for 2 h at room temperature