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Confocal laser endomicroscopy (CLE) is a more recent method to directly assess intestinal barrier function

It activates two of the same three receptors (GLP-1 and GIP), missing only the glucagon pathway

This injection is given into the muscle since muscular tissue contains more blood supply

The sections were then incubated with primary antibodies against occludin (Abcam, dilution 1:100) and ZO-1 (ThermoFisher, dilution 1:100), diluted in PBS containing 0.2% BSA and 0.1% saponin at 4 C overnight, washed, and incubated for 1 h with Alexa Fluor 647 donkey anti-rabbit IgG secondary antibody (Invitrogen, dilution 1:400)