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In line with patient studies, cellular and mouse models of ALS also showed high levels of ROS, along with elevated oxidation of membrane phospholipids (HNE, MDA), DNA (8-OHdG), and proteins (protein carbonyl groups, 3-NT) [94, 109,110,111]

However, standardizing these techniques for widespread clinical use remains a challenge, and further validation of these biomarkers is necessary before they can be incorporated into routine clinical practice

Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

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