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I mention the above in case anyones deliberating between trying Tongkat Ali or Fadogia Agrestis

Briefly, bacterial pellets were lysed in buffer (150 mM NaCl 2 , 50 mM Tris, 10% (v/v) glycerol, 1 mM phenylmethylsulfonyl fluoride (PMSF) at pH 7.4 by bead-beating as described for the ATP assay