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Description

The 5 targeting region was digested with Kpn I/ Hin dIII and cloned into Kpn I/ Hin dIII pL0001-digested plasmid

Gamma-aminobutyric acid regulates both the survival and replication of human beta-cells

To fully phosphorylate the C-terminal extension of UBE2H for ubiquitylation assays (which boosts its CTLH E3-dependent activity), it was coexpressed with the catalytic subunit of the kinase CK2 (CK26His) 39 and subjected to anion-exchange chromatography with the HiTrap Q HP column (Cytiva) before final SEC in buffer A