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Pull back the plunger to the mark of the amount of water you plan to use (e.g., the 1mL or 2mL mark)

University of North Carolina Press

Glycerol release was measured under basal and forskolin-stimulated conditions using a colorimetric assay kit (Sigma-Aldrich)

In addition, SHYS reduced the elevated total iron concentration and FTH/FTL ratio in the renal tissues of these mice, indicating relief of the iron overload