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In our LC-ICP MS experiments K D values (determined by NTA and His) in 5 mM HEPES, 50 mM NaCl (data not shown) were similar to those in 50 mM HEPES, 50 mM NaCl, which allows to conclude that HEPES at 50 mM level does not compete with HSA, NTA and His for Cu(II) binding or ternary HEPES complexes are formed with both CuHSA and CuNTA

The package contains a lot-specific Certificate of Analysis covering HPLC purity, LC-MS identity, ICP-based copper content, and the LAL endotoxin reading, alongside a Safety Data Sheet and a brief handling reference

Additional research is required to verify these advantages in humans

But if you're hoping to make the process smoother for both of you next time, a few simple adjustments can go a long way