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They demonstrated that antibody molecules (protein which bind to foreign molecules and target them for clearance or further immune response) can also generate ROS when they bind to their target

After resolving samples on SDS-PAGE and nitrocellulose membrane transfer, western blot normalization was performed with the Licor Revert 700 Total Protein Stain Kit as described in their protocol

The mean values were analysed

Moreover, GI comorbidities such as IBS are frequently observed in CFS/ME, further supporting the hypothesis that gut microbiota imbalances are not merely a consequence of illness but may actively contribute to its pathogenesis (He et al., 2023)