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This method comprises administering to the subject an effective amount of a pharmaceutical composition comprising a pharmaceutically acceptable carrier or diluent and a compound having the structure of formula (I): R 1 and R 2 , are independently selected from the group consisting of no atom, H, D, O, halo, C 1-6 alkyl, C 1-6 alkyl-aryl, C 1-6 alkyl-heteroaryl, C 1-6 alkenyl, C 1-6 alkenyl-aryl, and C 1-6 alkenyl-heteroaryl, wherein the C 1-6 alkyl, C 1-6 alkyl-aryl, C 1-6 alkyl-heteroaryl, C 1-6 alkenyl, C 1-6 alkenyl-aryl, and C 1-6 alkenyl-heteroaryl may be optionally substituted with an atom or a group selected from the group consisting of halo, deuterium, C 1-4 alkyl, CF 3 , and combinations thereof

While this study provides significant insights, it has several limitations: (1) Restricted age range: The study included only healthy adults aged 20 to 79 years, excluding specific groups such as pregnant women, infants, and adolescents

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2 and 3), suggesting the essential role of the Ang II/AT 1 receptor system in DPN development