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2017 Cell study used 5 mg/kg intraperitoneal injection three times per week in aged mice, with treatment periods of several weeks the three-times-weekly dosing suggests the peptide maintains sufficient functional concentrations for at least 48-72 hours per dose (PMID: 28340339)

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Lead is a known potent inducer of oxidative stress and inflammation: it impairs glutathione and antioxidant enzyme activity, generates excess reactive oxygen species, and disrupts nitric oxide signaling, leading to endothelial dysfunction and a pro-inflammatory state ( Importantly, many of lead's cardiovascular effects are indirect , acting via established AF risk factors