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The gene level expression was evaluated using reverse transcription-polymerase chain reaction and protein expression was studied using Western blot analysis

& Kristal, B

Peptides are short chains of amino acids that act as signaling molecules, helping regulate metabolism, appetite, fat storage, inflammation, and tissue repair at a cellular level

Protein and lipid pellets were discarded, while 10 L injections of supernatants were analyzed via a Vanquish UHPLC system (Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific) utilizing an untargeted acquisition as previously reported 18