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In step 5, C 5 H (hydride ion: H - ) of IN4 is transferred to the carbon of the amide neighbor of the cofactor NAD M to form the IN5 and reduced NAD + (NADH) via the transition state [TS5], which requires 32.2 kcal/mol of activation free energy

An 8-week-old germ-free female C57BL/6J mice were housed in an isolator (CEP Standard Safety, McHenry, IL, USA) with open cages in the University of Alberta Axenic Mouse Research Unit

The kidneys were the major route of excretion for (14)C-DTPA after IV injection

(4) Because selenium can reduce thyroid antibodies, I believe this is why it can help with thyroid-related anxiety