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Description

The Keap1 protein releases Nrf2 during oxidative stress for nuclear translocation and gene activation

Mixtures of the designated purified proteins were incubated with glutathione Sepharose beads or Ni-NTA beads in pull-down buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, 1 protease inhibitor) at 4 C for 2 h

Conducting high-quality, multicenter RCTs with standardized protocols and extended follow-up will be essential to validate efficacy, clarify mechanisms, and promote wider, evidence-based integration of TCM into comprehensive tinnitus management strategies