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The cells were then stained with Hoechst and mounted with Fluoromount-G (SouthernBiotech, 0100-01), LAMP1 (Santa Cruz, sc19992), TLR9 (Thermo Fisher, PA5-20203), TLR7 (Novus Biologicals, NBP2-24906), Rab5 (BD, 610281) and Rab7 (Scigen, AB0033)

In contrast, when the intestinal microecology is disturbed, the host is susceptible to pathogens, and the spleen and stomach malfunction, leading to qi and blood deficiencies, as well as poisonous metabolites

Depression and Mood Changes: B12 is involved in the synthesis of neurotransmitters , and deficiency can contribute to mood changes and symptoms of depression