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The full nrf2a coding sequence fused to the mKO2 protein via the T2A peptide was synthesized by Genewiz Company and was subsequently cloned into a pME vector to create a pME- nrf2a _T2A_mKO2nls vector

The current longitudinal literature is sparse and mixed, with some but not all studies reporting neurometabolite differences 14,15,16,17,18

In our study, as the intracellular -GC level was only increased slightly compared with that of original strain W303-1b when F pathway was introduced, we artificially introduced a flexible six-glycine linker between the two enzymes of G pathway as well as P pathway to improve the coupling efficiency by mimicking the native domain fusion of GshF

Supplementing glutathione