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10.1016/j.immuni.2014.09.008 4

ROS formation is evaluated via the use of 2,7-dichlorofluoroscein, a fluorescent probe, to detect ROS in biological systems following the addition of manganese (Chen et al., 2010[85])

This is a compound where the preclinical signal is strong, but the clinical confirmation is incomplete

The results showed that the slopes of the spiked BSA solution closely matched those of the spiked extraction solvent (no matrix), while the slopes of the spiked A549 cell extract differed significantly, indicating strong matrix effects at a cell density of 0.2 10 cells per 500 L (examples are shown for GSH and Glu in Fig